Miniaturized Automated Library Preparation Using the NEXTFLEX™ Ultra HT WGS Kit by Revvity

Abstract

Whole-genome sequencing (WGS) applications increasingly require library preparation workflows that can support high sample throughput while maintaining consistent library quality, automation compatibility, and efficient reagent use. As WGS adoption expands across population-scale, agricultural, translational, and routine genomic applications, laboratories are under increasing pressure to reduce total workflow cost without compromising data quality or operational robustness. This is particularly important for laboratories processing large sample cohorts, where workflow scalability and cost efficiency must be balanced against the need for reproducible sequencing-ready libraries.

The NEXTFLEX™ Ultra HT WGS Kit is a new Revvity solution designed to support highthroughput WGS library preparation. To evaluate its suitability for miniaturized automated implementation, the workflow was tested at one-quarter reaction volume in a 96-well plate format using the I.DOT™ Non-Contact Dispenser and G.PURE™ GEN 2 NGS Clean-Up Device. The study assessed whether the miniaturized workflow could generate libraries of suitable concentration and size distribution while maintaining clean negative controls and avoiding detectable adapter or primer-dimer contamination.

This application note summarizes the miniaturized library-preparation workflow and presequencing quality-control results, sequencing performance, coverage uniformity, and genotype concordance using a well-characterized Mimix™ reference standard.

Overall, the results demonstrate high dispensing precision, efficient DNA cleanup, and supporting the suitability of the G. PURE for automated, bead based NGS library preparation workflows